Sentence examples for used two steps of from inspiring English sources

To obtain the cDNA of NM_028662 and NM_134060, mouse genes that were identified in this study, and to create recombination sites for the GATEWAY™ cloning system (Invitrogen), we used two steps of attB adaptor PCR and prepared attB-flanked PCR products.

Since DELLA proteins do not directly bind to DNA, we used two steps of cross-linking during tissue fixation.

Furthermore, we used three steps of phenol/chloroform purification to eliminate remanent proteins.

Using two steps of statistical factorial design (Plackett-Burman and Central Composite) to optimize the production medium enhanced α-amylase production by 10.3-fold.

In this study, multiplex nested RT-PCR (mnRT-PCR) was applied to simultaneous detect multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza, infectious bronchitis and Newcastle disease virus using two steps of amplification.

These were then further developed using two steps of subcloning, which finally led to the subclone mAB328C6D11 (mAB328) that shows a high specificity for Jmjd6 staining (Figure 2A).

The main strengths of our study include thorough patient examinations with gold standard methods for measurement of insulin sensitivity, and using two steps of hyperinsulinemia.

A SU-8 structure using two step of lithography is formed to fix the polished fiber, which helps control the cavity length precisely.

In general, we can get the initial scale parameter λ just using three steps of iteration.

This improvement was achieved by using four steps of LMS pretreatment and NaOH washing [72].

Here, we developed a Bead-based Normalization for Uniform Sequencing (BeNUS) procedure using three steps of bead purification.