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After the combo chip was made we used "trimmed mean" to scale the chips and filter out the genes with detection call "Absent in ALL" the chips and absolute fold change less than 2 in all of the possible pairwise comparisons.
We used trimmed EST length as transcript length.
As a general hedge against such errors, we used trimmed means to estimate ν, throwing out the 10% largest per-nucleus or per-nucleus-pair estimates before averaging.
Since only one biological samples was sequenced, in order to avoid possible high false positive rates we first used trimmed mean of M values (TMM) to standardize data processing [ 48], then took advantage of DEGseq to analyze differentially expressed genes; the analysis conditions were qvalue <0.005 & log2 (foldchange) >1 [ 49– 51].
Similar(55)
Table 3 shows results for Egger intercept or FAT-PET regressions for price and income elasticities, estimated using trimmed samples.
In the present work, a new spline based topology optimization using trimmed spline surfaces and the isogeometric analysis is proposed.
The CAD models of relevant complex parts are usually defined using trimmed NURBS (non-uniform rational B-spline).
De novo assemblies for each tissue type were performed by Trinity software [ 51] using trimmed reads from Illumina sequencing.
The runs were normalized using trimmed mean of M-values (TMM) [ 36] and log2 transformation, centering and scaling.
The count data were normalised across libraries by using trimmed mean M (TMM) values of the default methods edgeR.
Not the content, but the pacing and length; it could use trimming.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com