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An EnVision Detection kit (GK500705: Gene Tech, Shanghai, China) was used to visualize tissue antigens.
We demonstrated in our article how this information can be used to visualize tissue heterogeneity based on a number of known molecular markers.
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Diaminobenzidine (DAB, Zhongshan Biotechnology, Beijing, China) was used to visualize the tissue slide and the sections were counterstained with haematoxylin.
The LSAB + system (DAKO Japan, Kyoto, Japan) was used to visualize the tissue antigens lipocalin-2 (LCXCL1 CXCL1 and CXCL9, according to the manufacturer's instructions.
Fluorescent antibodies can be used to visualize the tissue and cellular distribution of receptors with a far greater lateral and axial resolution than offered by autoradiography [ 114, 115].
Because of these advantages, the CARS imaging technique has been used to visualize various tissue structures, such as lung and kidney [ 12].
OCT is a non-destructive, rapid, three-dimensional imaging tool that has been used to visualize biological tissues and other materials.
A low concentration of iodine-based buffered formalin solution with a long staining period was used to visualize soft-tissue structures in a large goose head.
Iodine-based contrast media are usually classified as ionic or nonionic and as monomeric and dimeric and are commonly used to visualize vessels, tissues, organs, and the urinary tract.
In addition to its suitability for studies on sea urchins, MRI can also be used to visualize the soft tissue anatomy of other invertebrate as well as vertebrate taxa [ 3].
qBEI, a well established and validated method [28], was used to visualize the mineralized tissue with a spatial resolution of 1 μm per pixel, to quantify the local bone mineral/Ca content and select the regions of interest for SR μ-XRF measurements in the bone tissue.
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