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The T7 RiboMAX Express RNAi System Kit (Promega, Madison, WI, USA) was used to synthesise siRNA.
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The sequences used to synthesise the siRNAs are listed below: BRCA1-siRNA 5′-AATGCCAAAGTAGCTAATGTA-3′ Control-siRNA 5′-CGATAGATACACAGATTGAAT-3′ BRCA2-siRNA 5′-AACTGAGCAAGCCTCAGTCAA-3′ Oestrogen receptor- α activity was measured via transient transfection assays, using an oestrogen-responsive luciferase reporter (Fan et al, 1999b, 2001a).
Block copolymers can be used to synthesise different nanostructures due to their fitting size and shape.
A modified microwave method was used to synthesise the TiO2/MWCNTs hybrid nanocatalysts.
In this study, crude extracellular keratinase from B. safensis LAU 13 was used to synthesise AgNPs.
The VPP process was used to synthesise films of PEDOT on oxidant-coated substrates.
Cementitious materials and graphene oxide were used to synthesise the IHSSC-CA.
Oxidised forms of carbon, in particular CO2, can be used to synthesise energy-rich organic molecules.
It is then used to synthesise graphically a complete exchanger network, achieving the Energy Targets.
A fuzzy model is used to synthesise a new indirect adaptive fuzzy sliding mode controller.
This reaction releases energy that can be used to synthesise ATP and drive metabolism.
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