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In vivo microscopy of the spinotrapezius muscle was used to study systemic arteriolar dilator responses to the Ca2+ ionophore A23187, administered by ejection via pressurized micropipette into the arteriolar lumen.
We used an updated genome-scale metabolic network model of Saccharomyces cerevisiae, iMM904, to investigate how changes in the extracellular metabolome can be used to study systemic changes in intracellular metabolic states.
In vivo microscopy of the spinotrapezius muscle was used to study systemic arteriolar responses to intraluminal infusion of the Ca2+ ionophore A23187 or iontophoretic abluminal application of the adrenergic agonist phenylephrine (PHE).
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In the field of autoimmune disorders, conventional printed arrays have been used to study RA, systemic lupus erythematosus, multiple sclerosis, hepatitis and encephalomyelitis [ 29- 34].
The mouse model is widely used to study the mechanisms of systemic salmonellosis [ 16, 17].
In addition, the multi-tissue model was used to study diabetes: a pathology with systemic properties.
A similar arthroscopic-guided tissue sampling method and immunohistochemical analysis have previously been used to study the therapeutic effects of systemic infliximab therapy on the synovial expression of other important pro-inflammatory molecules [ 38, 47].
The systemic model of infection is widely used to study immune responses to LM.
We used this model to study systemic effects of sepsis but examination of brain lesions was not carried out [ 3].
With the advent of microarray technologies for measuring genome-scale transcriptional responses, there has been a renewed interest in using computational methodologies to study systemic responses [1].
Systemic inflammation will be measured in peripheral blood using several methods to study systemic activation of innate immune cells at four different levels: Expression of established and new markers on innate immune cells associated with preactivation.
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