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Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions.
The rat pheochromocytoma cell line (PC12) has been extensively used to study neuronal differentiation.
WGA protein is a trans-synaptic tracer that is used to study neuronal networks (Yoshihara 2002).
In utero electroporation is widely used to study neuronal development and function by introducing plasmid DNA into neural progenitors during embryogenesis.
Therefore, Mn2+-enhanced MRI (MEMRI) has been used to study neuronal activity and to visualize neuronal connectivity in different animal models (Silva and Bock, 2008).
fMRI is regularly used to study neuronal activity in humans because of its non-invasive nature, which allows it to be used to study a broad range of participants, including patients suffering from pathologies such as autism.
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The experimental model of global cerebral ischemia (GCI) has been widely used to study delayed neuronal cell death of sensitive hippocampus CA1 pyramidal neurons, which occurs 2 4 days after the onset of reperfusion [16].
This cell line has been used to study the neuronal effects of estrogens [37], but the physiological relevance of an immortalized cell line can always be questioned.
Two major techniques used to study mammalian neuronal network activity in vivo, tetrode recording of action potentials and bulk-loaded calcium imaging [39], suffer from the inherent problem of cell type heterogeneity - the cell type identity of the recorded or imaged neuron is extremely difficult to determine.
This method is efficient not only for clustering cell body distributions but may also be used to study other distributed neuronal structural elements, including synapses, receptors, dendritic spines and molecular markers.
Functional connectivity magnetic resonance imaging (fcMRI) is a powerful tool for understanding the network level organization of the brain in research settings and is increasingly being used to study large-scale neuronal network degeneration in clinical trial settings.
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