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These and other data are used to study breast cancer risk factors and the effectiveness of breast cancer screening, diagnosis, and treatment.
The MDA-MB-231 cell line has been widely used to study breast cancer metastasis and is therefore an appropriate cell model for this screening (Kang et al., 2003; Minn et al., 2005; Bos et al., 2009).
Array-based comparative genomic hybridization, RNA expression profiling, and proteomic analyses are new molecular technologies used to study breast cancer.
We review the main models used to study breast CSCs and how they challenge the CSC hypothesis.
Protocols that were used to study breast tumour xenografts in mice were approved by Loyola University's Institutional Animal Care and Use Committee.
The protocols used to study breast tumor xenografts in mice were approved by Loyola University's Institutional Animal Care and Use Committee.
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MCF10A cells are a spontaneously immortalized breast epithelial cell line that have been extensively used to study normal breast epithelial function [ 31].
Although these technologies were originally used to study invasive breast cancer, they are now being extended to pre-malignant and pre-invasive disease, facilitated by other new technologies such as microdissection and nipple duct aspiration, ductoscopy, and ductal lavage.
Of interest and attesting to its robustness, the free-floating sphere culture system is also used to study amongst others, breast cancer stem cells [11], [19].
For example, mammary luminal epithelial cells (MECs) are used to study mechanisms of breast cancer, but the proliferation of primary cell cultures is highly limited.
The last two articles in the series deal with molecular techniques used to study pre-invasive breast lesions.
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