Sentence examples for used to replace wild from inspiring English sources

This site, in conjunction with the naturally occurring EcoR1 site at 1063, was used to replace wild type E7 with codon-modified E7 genes.

T. graeca can be bred in captivity, and captive offspring could thus be used to replace wild populations (or be used for the pet market to protect wild animals from illegal trade).

Purified PCR fragments were digested by SacI and BamHI and used to replace wild-type hbpR in the egfp expression plasmid pHBP269A0 to test the inducibility of the mutants for biaromatic compounds [30].

Each of the four mutant toxR alleles conferred ICP2 resistance and abrogated expression of OmpU when used to replace wild-type toxR in a clean genetic background.

The msh2::kan allele (generously provided by Clifford Zeyl), a complete deletion of the coding sequence of the mismatch repair locus MSH2, was used to replace wild-type MSH2 in YPS3343 using a standard transformation protocol [ 53], producing isogenic strain YPS3460.

An internal EcoRI restiction site and the BamHI restriction site were used to replace the wild type sequence in pIRESneo3 GFP-ESCO2 by the mutant sequence.

The second-round products were treated with appropriate restriction endonucleases and were used to replace the wild-type fragments.

In case of successful mutation, the A-domain gene regions were recovered by SacI and BamHI digestion, and used to replace the hbpR wild-type A-domain sequence on pHBP269A0.

In order to characterize the function of Bar1 in F. graminearum, a split-marker approach was used to replace the BAR1 gene in wild-type strain PH-1 with a hygromycin phosphotransferase marker from plasmid pUCH2-8.

Genetic crossing with the wild-type parasites can be used to replace the mutated DNA polymerase δ with the wild-type enzyme.

After digestion with these enzymes, the resulting DNA fragments were used to replace the SacII– SnaBI fragment containing wild-type NES-A in plasmid pRR2.