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A Gaussian filter 0.2 micrometer wide was first used to remove variation in local intensities smaller than spots of single mRNAs, see Figure 7B.
Partek® Genomics Suite™ 6.4 (Partek Inc., St . Louis MO) was used to remove variation in the data due to batch effects.
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The regression defined in Equation 1 was then used to remove variations in foF2 related to the solar activity effect, allowing monthly absolute deviations (defined as ∆foF2 = foF2 - foF2′), which can potentially reveal long-term trends in foF2, to be calculated (Figure 2b).
A stringent quality control and inter-array normalisation procedure was used to remove confounding variation due to experimental factors, resulting in a normalised data matrix of methylation scores (β-values, 0<β<1) across 383 samples (148 healthy, 113 pre-treatment (preT) ovarian cancer cases, 122 post-treatment (posT) ovarian cancer cases) and 25,642 CpG sites (Materials and Methods, Figure S1).
The absolute glucagon change from baseline was used to remove intrasubject variation in baseline levels.
A 365-day (1-year window) running average can be used to remove seasonal variations of GEC as well as the shorter-term variations of GEC and EUV (including the solar rotation variation).
This smoothing step was used to remove individual variations in gyral anatomy and to render data more normally distributed by the central limit theorem.
The natural spline smoothing function was used to remove the small variation while maintaining the major trend of each variable, i.e. to make them smoother.
The models can then be used to remove environmental and operational variations so that change detection algorithms signal the occurrence of damage alone.
A reference electromagnetic sensor was used to remove the dependence of relative position variation between the FG and the template grid.
A Carulite-200® catalyst was used to remove peroxides from ambient air to blank the system to account for variations in the ambient air matrix.
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