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BlastClust [ 15] was subsequently used to reduce sequence redundancy within the dataset.
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An implementation of the Amplicon Noise algorithm was used to reduce the sequencing error incurred with pyrosequencing (Quince et al.2011).2011
Three strategies were used to reduce the effect of sequence errors, one being more aggressive than the other two.
Pooling strategies have been previously been used to reduce costs for BAC sequencing [ 44].
For each PCR step, a high-fidelity DNA polymerase with 3ʹ−5ʹ proofreading ability and high processivity was used to reduce the introduction of sequencing errors.
To solve Equation 1, the quasilinearization technique is used to reduce Equation 1 to a sequence of linear problems.
DNA from low passage numbers was used to reduce the error rate from sequence alterations and chromosomal loss because of EBV transformation (21, 22).
More recently, exome capture was used to reduce the representation of genome sequencing for X-QTL mapping with relatively low sequencing depth (95−366×) (Chevalier et al. 2014).
Enrichment protocols have been used to reduce these costs by focusing sequencing efforts on DNA clones that are likely to contain a particular repeat motif as done by Budak and co-workers.
A cut off phred quality score of 20 was used to reduce false positives due to sequencing errors, ensuring that only high quality bases with an error rate of ≤ 1% were eligible for mutation findings.
The paper shows advanced spatial, temporal and spatio-temporal filtering techniques which may be used to reduce noise effects in photogrammetric image sequence analysis tasks and tools.
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