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Ms. Hart, who moved from Denver in late August, used to pull down the blinds and turn on the air-conditioner in her temporary housing in Chelsea to block out the city at night.
Yesterday, workers in baskets, lifted by cranes into the air, set up cables that will be used to pull down the last standing pieces of 1 World Trade Center, the north tower.
Where indicated Nickel-beads were used to pull down His-tagged Ub proteins.
For the biotin pull-down assay, biotin-labeled H3 peptides were used to pull down the PHD1KDM5B protein in order to determine the binding affinities.
These streptavidin tagged micro beads or magnetic beads are used to pull down cell membrane protein or protein complexes from cell fractionation.
GR antibodies were first used to pull down GR-associated DNA fragments.
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Protein A/G beads were used to pull-down PCAF.
Protein A/G agarose beads were used to pull-down E2F1.
Anti-RIG-I or anti-IPS-1 antibody (Sigma, St Louis, USA) were used to pull-down the immunocomplex.
Bacterially expressed and purified GST-tagged TRIM24 mutants coupled to GSH beads were used to pull-down LNCaP protein lysates.
Co-immunoprecipitation was performed using 2 μg of antibodies for 1 mg of total cell lysate and protein G sepharose (Sigma-Aldrich) was used to pull-down immunocomplexes followed by washing by NP-40 buffer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com