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In addition to the therapeutic milk F-75 being airlifted in over the next few days, UNICEF is distributing one ton of a mineral and vitamin complex which will be used to prepare additional therapeutic milk – enough for 1000 children for three months.
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These dissected samples were used to prepare 17 additional small RNA libraries that were sequenced using SBS.
The following E. coli laboratory strains were used to prepare SLiCE extracts (Additional file 1: Table S1): DH10B [ 45], JM109 [ 46], DH5α [ 47], XL10-Gold (Agilent Technologies, Santa Clara, CA), Mach1 T1 (Life Technologies, Carlsbad, CA) and SURE2 (Agilent Technologies, Santa Clara, CA).
For some libraries used to prepare the first array, an additional 14 lines from non-cultivated species of Avena were used.
Cleaning validation need to be performed both in case the automated system is used to produce a single radiopharmaceutical (e.g. [18F]FDG) and in case it is used to prepare different RPs, which may pose additional problems of cross contamination.
The methods used to prepare this library are presented in Additional file 1.
Additional aliquots were used to prepare smears that were air-dried and stored for later analyses of sperm morphology [ World Health Organization (WHO) 1999].
The synthetic chemistry platform that was used to prepare KAM1 was modified to synthesize these additional analogs, which were then tested for biochemical and cellular activity.
After RNA quality control (Additional file 2: Table S2), 16 samples were used to prepare two STRT libraries using 3 technical replicates, each containing 10 ng total RNA (Additional file 3: Table S3).
20 Details of the methods used to prepare and validate the dataset can be found in additional publications.
Oligonucleotides used to prepare clones and probes for Northern blots are listed in Additional file 2: Figure S9.
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