PIP-strips supplied by Echelon Biosciences, Inc. (Salt Lake City, Utah, USA) were used to perform phosphoinositide binding according to the supplied protocol.
DiffBind was then used to perform edgeR differential binding analysis.
The array was used to perform a protein protein binding assay using the SUMO2 paralog.
It can then be used to perform visualization tasks like binding results of a query to a DataGrid control, create views of the data in WinFS itself, or just extract the query string.
Thus, an extract enriched with CS-bearing PGs was purified from whole mouse brain and this extract was used to perform a solid-phase binding assay with various forms of Aβ (Fig. 2a and b).
For example, an NMR-based approach was used to perform a stepwise replacement of binding events in the PPI between the AVPI natural tetrapeptide and the BIR3 domain of XIAP.
High-throughput (HT) assays, based on multi-well plates traditionally have been used to perform systematic measurements of in vitro binding, enzymatic activity or gene expression.
We transfected NIH-3T3 cells with T352A or F327A mutants of the full-length TrkA-coding construct, selected stable transfectants, and used them to perform binding experiments with tritiated MT2, as described above.
To determine which component of the agarose beads was responsible for the binding to AID, we performed binding assays using agarose coupled with protein G, protein A, calmodulin or (irrelevant) antibody.
