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The methods used to isolate single spores and to store the resulting isolates have been described elsewhere (Feng et al. 2007; Zeng et al. 2009).
Currently, the alternative methods used to isolate single cells from abundant populations include serial dilution, mechanical micromanipulation, laser capture microdissection (LCM), fluorescence activated cell sorting (FACS), and microfluidics [11, 12].
Conventional spike sorting approaches (Offline Sorter; Plexon) were used to isolate single unit activity.
Clonal selection was used to isolate single cell-derived sublines of the M006XLo line.
Bacterial subcloning was then used to isolate single copies of the promoter regions for subsequent sequencing and analysis.
The enzymatic dispersion techniques used to isolate single ventricular myocytes from neonatal Wistar rats have been described previously in detail [ 13].
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MACS is another method used to isolate single-cells [26].
Out of these 50 clones, 34 positive phage clones (7 highly hyphal specific and 27 moderately specific to either yeast/hyphal form) and 2 clones (H-15 and H-41) showing no significant binding, were used to isolate single-stranded DNA [11].
Mechanical tissue disruption and 70 μm filtration were used to isolate single-cell suspension.
route and spleens and lymph nodes draining the respiratory tracts (mediastinal lymph nodes, MLN) and the vaginal tracts (iliac lymph nodes, ILN), respectively, were collected on day 7 p.i. and used to isolate single-cell lymphocyte populations, as previously described [ 20].
In these cases, preparative-GC (prep) or preparative multidimensional gas chromatography, suitably designed with necessary trapping and heart-cutting devices in a conventional GC, can be used to isolate a single compound.
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