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In addition, to minimize cross-hybridization, at least eleven probe pairs (positive match and mis-match oligonucleotides) are used to interrogate each taxon at at least two discriminatory loci on the 16S rRNA gene.
The terms used to interrogate each databases are presented in the Supplementary Table 1.
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Biological replicates from Florida were used to interrogate a single microarray slide each.
Each fraction was then used to interrogate high-density microarrays and an estimate of the translational efficiency of each mRNA was defined here as the ratio of the hybridization signal in fraction-W over that of fraction-U.
MS/MS spectra generated from each digest were used to interrogate a "SuperWheat" database.
RNA was extracted from approximately 1,500 embryos from each sample and was used to interrogate a 2,620 feature (1,310 double spotted unigenes) custom array [36], [48].
To search for additional matches that might indicate the presence of gene families, each annotated gene was used to interrogate the D. pulex genome assembly.
Equal amounts of total RNA were pooled from 7-10 mineach each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (n = 1 per group).
Equal amounts of total RNA from individual samples were pooled from mice in each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (Affymetrix, Santa Clara, CA, USA).
Briefly, an anchor oligonucleotide is hybridized to one of the four adaptors, and four probes, each carrying a spectrally distinct dye, are used to interrogate a base position adjacent to the adapter.
"It's used to interrogate poor brown and black young men.
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