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Transgenic flies carrying an insertion of this targeting construct on the 3rd chromosome were used to generate precise targeting events as described [27].
Furthermore, CRISPR was used to generate precise point mutations in two targeted genes simultaneously in the presence of single-stranded DNA oligonucleotides with point mutations as templates for HDR repair.
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Thereafter, numerous Greek and Middle Eastern objects were imported into Etruria, and these items, together with Etruscan artifacts and works of art displaying Greek or Oriental influence, have been used to generate relatively precise dates along with more general ones.
Bootstrapping with 500 replications was used to generate more precise standard errors than conventional OLS regression.
Our photoactivation system can be used to generate spatially and temporally precise Ca2+ signals and to engineer synthetic proteins that respond to specific Ca2+ signals.
Photo-fabrication techniques have already been used to generate 3D environments with precise architectures and heterogeneous composition, through a multi-layer procedure involving the selective photocrosslinking reaction of a light-sensitive prepolymer.
In the analyses, these weights (and replicate weights) were used to generate appropriate standard errors from which precise 95% confidence intervals were estimated.
The precise UNIX commands we used to generate the predictions are also indicated in Figure 3.
A strong dominant suppression of variegation was observed for all four dATRX alleles (Figure 3) in comparison to both yellow-white flies (wt) and a precise excision of the P-element used to generate the mutants.
Simultaneous confocal scanning laser ophthalmoscopy is used to generate high-resolution images of the retinal surface, thereby providing precise location information of each A-scan within a cross-sectional SD-OCT image.
However, this method does not allow precise control of important ligation conditions such as reaction stoichiometry and it cannot be used to generate stable thioester proteins for subsequent conjugation to cysteine-functionalized surfaces.
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