Sentence examples for used to generate mutants from inspiring English sources

Importantly, to proof a broad applicability in other fungi the same strategy was used to generate mutants in the filamentous ascomycete Aspergillus nidulans.

The tool we used to generate mutants in these case studies is called Milu [33] (version 3.211).

The primers, each complementary to the opposite strands of the vector, were used to generate mutants.

The following oligonucleotides were used to generate mutants: RGD mutant (TatR 78 K,D 80 E) 5'-CCC GCC TCC CAG TCC AAA GGG GAA CCG ACA GGG CCC-3'; basic mutant (TatR 53,54,55,56,58,59 A) 5'- GGC ATC TCC TAT GGC GCG AAG AAG GCG GCA CAG GCA GCA GCA GCT CCT CAG GAC -3'; CD26 binding domain mutant (Tat21 101) 5'-GGG GAA TTC CCC TGG AAG CAT CCA GGA AGT CAG CAG3'.

Site-directed mutagenesis was used to generate mutants of gal-7.

Site-directed PCR mutagenesis was used to generate mutants of PGBD5 and piggyBac, according to manufacturer's instructions (Agilent, Santa Clara, CA, United States).

The QuikChange Site-Directed mutagenesis Kit (Stratagene) was used to generate mutant SVOP constructs.

Primers 1 and 2 were used to generate mutant M-1.

Recently, physical mutagenesis such as heavy ion irradiation (HII) has been used to generate mutant populations of model, crop and ornamental plant species [ 16- 18].

Heterozygous mating was used to generate mutant embryos of desired genotypes (determined by PCR reactions) and specific stages described in the text.

PCR with primers introducing XbaI and MluI restriction sites flanking the reading frames was used to generate mutant or chimeric vpu and nef alleles for cloning into the pCG vector.