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Because binding takes place in a constant flow environment, the technique can be used to extract binding constants and affinities.
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The fraction of captured DNA was plotted as a function of protein concentration and a non-linear curve-fitting algorithm was used to extract equilibrium binding constants (Kd values) from the data.
Fits to the hyperbolic model used to extract the binding and inhibition parameters in Table 1 are shown.
A distance threshold of 4.5 Å was used to extract the binding sites from the remaining 30 956 protein ligand complexes.
Different clustering algorithms were then used to extract the present information on binding site shape differences.
The crystallographic structure of the ovine COX-1 complexed with celecoxib (PDB [38] ID: 3KK6 [39]) was used to extract the residues in the binding site.
IC50 values from competition assays have been used to extract a direct measure of binding affinity (K i) for a given target.
Unsupervised co-inertia analysis (CIA) was used to extract differentially represented transcription factor binding sites in the promoters of genes regulated by TGFβ, CCN2 or both together in HMCs.
Similar to the ChIP-seq method [17], since methyl-binding proteins were used to extract the methylated GC-rich DNA, we observed significant enrichment of sequence reads at CGI regions compared to other genomic regions.
Three different methods are used to extract them.
The pull used to extract quarters from your pockets.
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