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Three main techniques are used to excise sebaceous cysts: conventional wide excision, minimal excision, and punch biopsy excision.
For sampling, the bottom portion of a 55 mm Petri plate was used to excise a circular portion of the crust matrix (to a depth of ~ 1 cm) after wetting with a mist of sterile ultra-pure milli-Q water in order to make the crust supple to facilitate collection.
This gel was stained with Coomassie blue and was used to excise spots for protein identification.
The mice were killed by a brief exposure to a 100% CO2 gas atmosphere (to induce narcosis) and a midline abdominal incision was used to excise the ileum.
Five endogenous restriction sites in rNav1.2α ORF were used to excise DNA fragments coding for the four channel domains (DI: XhoI/XmaI, DII: XmaI/BglII, DIII: BglII/BstEII, DIV: BstEII/PacI).
A sterile razor blade was used to excise the bands.
Similar(31)
Forceps and a scalpel were used to carefully excise the white tissue just behind the root cap containing actively dividing mitotic cells.
Lastly, we demonstrate that this optimized PB1 endonuclease can be used to efficiently excise an herbicide resistance marker from transgenic Arabidopsis plants when the marker is flanked by recognition sequences for the enzyme.
The highly directional and tightly regulated recombination reaction used to site-specifically excise the bacteriophage λ chromosome out of its E. coli host chromosome requires the binding of six sequence-specific proteins to a 99 bp segment of the phage att site.
Subsequently, we used Int* to excise the tester and reconstitute the attP sequence at desirable landing sites.
To test this concept, we expressed FLP under a heat shock promoter and used it to excise a termination cassette upstream of the GFP coding sequence.
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