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1H NMR spectroscopy was used to evaluate binding of the oxoanions NO3−, ReO4−, CF3SO3−, H2PO4−, HSO4−, CH3COO− and C6H5COO− to the receptor.
Cyscore was used to evaluate binding affinity to TINAGL1, [ 45].
Concentration-dependent DNase I footprinting was used to evaluate binding of 2 and 3 to DNA sequences 2 5.
In this study, a single dose of 20 mg paroxetine was used to evaluate binding to SERTs in healthy controls.
In molecular docking process, the binding energy score is used to evaluate binding affinity between protein receptors and ligands.
Prototype enterohemorrhagic E. coli (EHEC) strain 86-24 and enterotoxigenic E. coli (ETEC) strain H10407 were used to evaluate binding to integrin α5 β1.
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A solid phase binding assay was used to evaluate the binding of the HA-Fc proteins to short synthetic receptor mimics bearing a single copy of lactosamine with terminal SA in the α2-3 (3SLN) and α2-6 linkage (6SLN) (Figure 2) [32].
The protein concentration was obtained by a dye-binding assay and immunoblot was used to evaluate the binding of the panel of mAbs produced.
A three-dimensional grid was created with 0.375 Å spacing with a 126 Å×766 Å×76 Å grid box by the AutoGrid program for the binding site of the receptor, which was used to evaluate the binding energy between the inhibitor and the protein.
PB and GB models are both used to evaluate the binding free energy in computational alanine scanning method and only GB model can be used to decompose the binding free energy on a per-residue basis.
Moreover, it could also be used to evaluate the binding affinity of NF-κB.
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