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As a negative control, PCR mix without DNA sample was used to ensure contamination free PCR product.
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The no reverse transcriptase and no template negative controls were used to ensure absence of contamination and of nonspecific amplifications.
Sample tracking controls were used to ensure against cross contaminations or erroneous loading in the array.
A reverse transcriptase negative control was used to ensure there was no DNA contamination.
The negative controls were used to ensure that there was no contamination but not used in the final calculation.
The results were determined from plates where no-template controls were used, to ensure that the reagents were contamination free.
Only the first three points (first ∼30 ms of systole) were used to ensure that there was minimal signal contamination from wave reflections (35).
A dissociation curve and negative control (cDNA reaction without RT-enzyme) were used to ensure primer specificity and lack of contamination.
The QIAGEN RNase-free DNasupplementent kit was used to ensure that the RNA was free from DNA contamination.
Loudspeaker radio is used to ensure communication.
Right now, it seems the very mechanisms we would be required to use to ensure no out-of-control contamination of Earth by alien microbes would destroy the very material we want to retrieve".
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