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Preservation of the vascular structure in decellularized porcine small bowel is used to engineer tissue with an innate vascularization.
GelMA is a versatile matrix that can be used to engineer tissue analogs ranging from vasculature to cartilage and bone.
This study shows that a sequential stimulus approach – cyclic stretching with delayed TGF-β1 supplementation – can be used to engineer tissue with desirable tensile and elastic properties.
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Microfabrication techniques have been used to engineer tissues with defined microscale structures, such as strips, squares, and wells, and have been successful in studies to understand single-cell- or tissue-like structure function relationships.
The poly-l/d-lactide 96/4 joint scaffolds are used to engineer fibrous tissue joints in situ for the reconstruction of metacarpophalangeal joints.
Furthermore, such alginate hydrogels can potentially be used to engineer cartilage tissues of scale to promote endochondral bone regeneration in large bone defects.
We envision that this method can be potentially used to engineer thick tissues when the microfluidic channels are perfused with culture medium to enhance nutrient and oxygen supply.
This in vitro culture system could be used to engineer functional salivary tissue in vivo.
Therefore, the dual delivery of DEXA and DHEA can be used to engineer inflammation-free tissue in the vicinity of the implant.
We discuss how the strategies used to engineer mature epithelial tissue models in vitro could be extrapolated to instruct the design of organotypic culture models that can recapitulate the structure of embryonic ectodermal tissues and enable the in vitro assessment of events critical to organ/tissue morphogenesis.
We envision that this simple yet versatile method can be potentially used to engineer 3D vascularized tissues.
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