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In its place, the enzyme trypsin was used to dissociate the cells and separate them.
In addition, other methods could also be used to dissociate the complex such as pH, enzymatic cleavage or temperature.
Two tasks were used to dissociate the physiologic role of empathy in producing generosity and altruism using monetary transfers.
In cases where it was important to keep all cell surface proteins intact, a solution of enzyme-free 0.2 g/l EDTA4Na and 0.2 g/l glucose in PBS was used to dissociate the cells.
This was followed by further perfusion with (non-recirculating) wash buffer to remove any remaining EGTA from the liver as the enzymes used to dissociate the liver are dependent upon calcium and magnesium for activation.
For regular cell cultures a 0.1% trypsin solution was used to dissociate the cells from the surface of the culture dish.
Similar(47)
Importantly, we propose that this energy is used to dissociate myosin from the actin filament, the most energy-intensive step in the reaction of the actin myosin functional cycle.
Twenty microliters of 2xSDS-PAGE gel loading buffer was used to dissociate proteins from the precipitates by boiling for 5 min.
The retinotopic nature of the spatial coding was used to dissociate effects that depend on the target position during stimulation and on the target position at interception.
This lysis buffer is routinely used to lyse cell membranes for the release of RNA/DNA and was also used to dissociate GAG associated with the cell pellet.
A Papain Dissociation System (Worthington Biochemical Corporation) was used to dissociate airway epithelial cells from the trachea as described [ 24, 25].
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