Immunochemical techniques are used to detect the expression of a particular antigen expressed either on the cell membrane, inside the cell, released by cell, or in the extracellular matrix.
A polyclonal antibody against AaIT that was expressed by E. coli BL21 (DE3) was derived from rabbits and used to detect the expression of AaIT in the Bb-AaIT or WT grown in CDA or in the infected mosquitoes by western blotting.
Thus, immunohistochemistry was used to detect the expression of IRF-1, p21 and BAK.
Monoclonal anti-β-actin (ACTN05, Neomarker, Fremont, CA) and anti-HIV p24 (Chemicon, Temecula, CA) antibodies were used to detect the expression of β-actin and Gag, respectively.
The following primers were used to detect the expression of rat GPR17: Fw 5'-GCTCTTCGCCTGCTTCTACC-3'; Rv 5'-GCGGACGGCTTTATTCTTGA-3' (PCR product: 610 bp, TA: 58.6°C).
The following primers were used to detect the expression of mouse GPR17: Fw 5'-GATGAACGGTCTGGAGGCAGCC-3'; Rv 5'-CTCACAGCTCGGATCGGGCAC-3' (PCR product: 1022 bp, TA: 61.4°C).
The purified antibody was used to detect the expression of MsDps2 in wild type M. smegmatis cultures grown under various conditions of growth, which included starvation, very late stationary phase and biofilm growth.
The following primers were used to detect the expression of specific genes: ST3GAL1 forward and reverse [10]; ST3GAL4 forward and reverse [11]; E14134 and E14135 for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA [12].
Immunohistochemistry was used to detect the expression of ILK.
Immunofluorescence was used to detect the expression of MMP-2, as described by the product's manufacturer.
After amplification, agarose gel electrophoresis was used to detect the expression of the genes.
