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The new method can be used to detect additional protein antigens recognized by γδ T cells in the future.
It was identified as a major horse allergen (accession: U70823), and has been used to detect additional expression in submaxillary glands and liver [40].
Membranes were stripped with stripping buffer (100 mM ß-mercaptoethanol, 2% SDS, 62.5 mM Tris-HCl, pH 6.8), washed with PBS-0.1% Tween 20, and used to detect additional proteins.
Third, subsequent lipiodol CT can be used to detect additional tiny HCCs with lipiodol deposition [ 6].
Cross_match and RepeatMasker were used to detect additional vector and adapter contamination after the sequence vector clip.
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For PCR3 35 cycles of PCR could be used to detect 104 cfu/ml, however additional bands appeared when increasing the cycle numbers and dominated in samples with 103 cfu/ml or less.
Two main phage-based approaches are used to detect M. tuberculosis [see Additional file]: (i) amplification of phages after their infection of M. tuberculosis, followed by detection of progeny phages using helper cells (plaque formation); and (ii) detection of light produced by luciferase reporter phages (LRP) by live M. tuberculosis [ 3].
Primers used to detect transgenes are listed in Additional file 1: Table S2.
The pattern used to detect HGT is shown in Additional file 2, Figure S4.
Gene specific mouse primers were used to detect leptin, adiponectin and resistin (Additional file 2: Table S2).
The gene-specific primers that were used to detect transcripts are listed in Additional file 1: Table S1.
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