Sentence examples for used to design additional from inspiring English sources

The nucleotide sequence of the recovered clone was determined and used to design additional primers for recovering the full-length gene (designated MmTKL1) from the genomic phage library.

This paper presents an uncertainty-directed sampling method that can be used to design additional samples for soil mapping.

Isolated DNA was sequenced with these primers and obtained sequence was used to design additional sequencing primers.

Once mapped, the genome sequence around the locus can be used to design additional genetic markers for fine-scale mapping, and to identify putative candidate genes using the genome annotation.

The genomic sequence obtained was used to design two additional primers such that two small amplicons of 120 200 bp could be amplified by quantitative PCR (qPCR) (Additional file 1: Table S3).

The Monte Carlo Neutron Particle code; version 5, (MCNP5) was used to design the additional safety rod at one of the inner irradiation sites with cadmium as absorber material and stainless steel as clad.

Primer3 software was used to design primers (Additional file 4: Table S4).

EST contigs were assembled in Vector NTI Advance 11.5, and their sequences were used to design primers (Additional file 2: Table S1) flanking full-length ORFs.

In a second step the determined sequences were used to design three additional PCR primer pairs (Table 2) bridging the gaps between rrnL- cox1, cox1- cob, and cob- nad4.

Primer Blast (NCBI) [ 28] was used to design primer pairs (Additional file 7: Table S4).

The longest PCR product was used to design primers for additional 5'-RACE.