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ClustalW [96] was used to calculate sequence identity between matches found between the testset of 1217 structural genomics proteins and their BLAST matches in SwissProt.
ISO1 (HM467823) was used to calculate sequence identities here.
The BLOSUM62 matrix was used to calculate sequence homology scores.
Here, only pairs of proteins with an E-value <10−5 are used to calculate sequence similarity.
With regard to the false-positive rates, there is only minimal difference across the various window sizes used to calculate sequence complexity.
On the one hand, detailed structural modeling of protein-DNA interactions has been used to calculate sequence dependent protein DNA interaction free energies [ 36].
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Good's method was used to calculate sequencing coverage [40].
The dynamic programming technique is used to calculate the sequence identity of two protein sequences of a gene pair.
The USEARCH algorithm was used to calculate global sequence identities of the V1-V3 regiof of 16S rDNA reference sequences for the species in the KJMOCK sample.
Read depth distribution was used to calculate average sequence coverage across the whole genome.
T-COFFEE (version 5.05) [ 45] was used to calculate pairwise sequence similarity between miRNAs.
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