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The criteria used to assign function to a CDS were (1) a minimum cutoff of 40% identity and 60% coverage of the protein length and (2) at least two best hits among the COG, KEGG, or nr protein database.
Reverse genetic approaches are often used to assign function to Plasmodium-specific genes.
Mapman was used to assign function to the Actinidia NRs and thus to their constitutive ESTs.
Notable examples are studies where the primary metabolism has been reconstructed and used to assign function to orphan genes.
The criteria used to assign function to a protein translated by predicted open reading frames (ORFs) were a minimum cut-off of 30% identity and at least three best hits among the NR, COG, KEGG and GO databases.
The Gene Ontology (GO) project [ 15] is an annotation framework that provides a standardized vocabulary that is used to assign function to uncharacterized sequences, based on three main categories: biological processes (BP), molecular functions (MF) and cellular components (CC).
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Predicted gene models were used to assign functions, EC numbers and map GO terms using the RAPYD functional prediction pipeline.
In particular, gaps in metabolic pathways can be used to assign functions to orphan genes.
The Gene Ontology database was then used to assign functions to genes [ 88].
A combination of BLAST (1e−5 cutoff), HMMER (1e−10 cutoff), and PRIAM (1e−30 cutoff) were used to assign functions to the sequences.
Guilt-by-association has been used to assign functions to proteins with unknown roles based on the functions shared by the majority of their direct neighbors (i.e. interaction partners) in protein interaction networks [ 25].
More suggestions(15)
used to quantify function
used to assign probability
used to evaluate function
used to analyze function
used to calculate function
used to infer function
used to assess function
used to disrupt function
used to modulate function
used to assign confidence
used to block function
used to predict function
used to augment function
used to measure function
used to enhance function
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