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Gene Ontology (GO) enrichment was used to analyze function and biological processes of the target genes.
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The C2C12 myoblast cell line was used to analyze the function of miR-133b during myogenesis.
Dual-Luciferase array was used to analyze the function of CDK11p58 in the regulation of VEGF promoter activity.
VIGS technology can be used to analyze gene function, and our experiments analyzed genes related to fruit color in detached fruit.
LMP2 deficient mice have been extensively used to analyze immunoproteasome function upon virus infection but exhibit no obvious phenotypic abnormalities at normal maintenance conditions [ 24].
RNAi screens are commonly used to analyze gene function in a variety of model organisms, the most popular ones being C. elegans and Drosophila [ 5, 6].
In this study, applied behavior analysis is used to analyze the function and impact of different behavior change interventions when implementing multi-professional teamwork.
To analyze the role of the p38 MAPK in AVE specification, we used SB203580, a specific inhibitor of the p38α and β [ 6], which has been used to analyze p38 function during preimplantation development [ 7, 8] and gastrulation [ 9].
On a cellular level, AFM and the dual pipette assay (DPA) have been used to analyze the function of Cadherins in regulating the de-adhesion force at cell cell contacts [ 25,57].
VIGS technology is a simple and powerful tool that has been widely used to analyze gene function in many plant families such as Solanaceae, Brassicaceae, Poaceae, Ranunculaceae, and Asteraceae (reviewed in [ 11]) and especially Fabaceae [ 7] where many species are difficult to transform genetically by other means.
Moreover, gene knockout and silencing techniques cannot be used to analyze domain functions and post-translationally modified protein functions.
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