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In this study we used the same tissue microarray cohort to evaluate the relationship between intra-tumoral macrophage infiltration and EMT.
Five experiments from about 1000 hits were selected that have used the same tissue in a similar developmental stage and of plants grown in almost similar experimental conditions as in our study (Additional file 2: Table S1).
The DA-depleted sample and the sample with L-DOPA administration employed the same tissue (since striatal tissue from 6-OHDA injured animals was used for controls and the test sample used the same tissue in the presence of L-DOPA).
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This study would have been much improved if we had always used the same tissue-sampling method.
To be able to compare RNase Dre1 and RNase Dre2 expression with that of RNase A genes, we used the same tissues utilized in the analysis by Cho and Zhang [ 16].
Due to the limitation of tissue material availability from the same individual, we have used tissue material from different individuals for the ChIP-seq experiments but used the same tissues along with a third set of tissues for RNA expression and found good correlation between histone peaks and gene expression, which suggests that similar mechanisms are present in multiple patients.
Also, other functional variants may affect the expression of PRNP and therefore carrying out confirmatory studies in these loci or other polymorphisms using the same tissue or other tissues in other populations would be useful.
The twins were autopsied at the same facility using the same tissue processing protocols.
Prior studies have focused on elucidating genes whose expression differs both between individuals and within the same individual, using the same tissue type.
Testing was done using the same tissue that had been used for gene expression arrays, and was performed for 31 genes.
Using the same tissue extract as described above, 20 µl aliquots were added in triplicate to wells of a 96 well UV transparent microtitre plate (Costar).
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