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None has yet said they have successfully reprogrammed cells, but most have not used the same cells Obokata used.
As a control we used the same cells complemented with the wild type gene (Fig 1A).
Since the secreted luciferase assay is non-destructive, we used the same cells to correlate the expression status of the miR-122 guide strand detected by Northern hybridization.
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We used the same cell in each of the following steps (shown in Figure 2).
We used the same cell-free supernatants as in the IP approach (compare Figure 3a lane 4 and 5).
As positive control we used the same cell line stably transfected with a WWOX expressing vector (PE01-WWOX) [ 10, 12].
To test the generality of this response, we used the same cell populations and induced cardiac differentiation by the hanging drop method.
To elucidate the role of potential upstream signaling molecules in canine cruciate ligamentocytes, we used the same cell-permeable inhibitors SB202190 and PD98059.
Therefore, we used the same cell lysates from the dual luciferase reporter gene assay for immunoblot analysis for detection of Nrf2, Keap1, and β-actin.
We used the same cell line, H460 and obtained consistent results showing that both miR-221 and miR-222 promoted liquid colony formation in H460 (Fig. 2A).
Both screens used the same cell line, reporters and experimental design, with the SRSF screen identifying 42 putative regulators of JAK/STAT signalling, 22 of which verified in a secondary screen and 16 verified with an independent probe design.
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