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In this study, we used the microbeam setting and AL cell system to extend the study to provide further evidence that cytoplasmic irradiation leads to elevated levels of ROS and RNS.
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The layout and methods for the cytoplasmic irradiation using the microbeam have been described previously (Hei et al, 1997; Wu et al, 1999).
Cell nuclei to be irradiated were randomly selected at the periphery or at the centre of the outgrowth and then positioned over the collimator using the microbeam stage.
Otherwise, use the "sun" setting.
Use the "cold" rinse setting.
Use the cold water setting.
(Use the "warm" setting).
Use the cool setting.
Use the Groups setting.
using the P.A.L.M MicroBeam instrument.
Laser catapult microdissection was accomplished using the PALM MicroBeam System (as above).
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