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In order to simplify the description of the functions of hundreds of FFs, we used the functional classification of Vogel and Chothia [ 23].
In order to get insights about these 84 genes, we used the functional classification tool of the DAVID database (http://david.abcc.ncifcrf.gov/ [40]).
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Therefore we performed a function enrichment analyses for the differentially expressed genes using the functional classification tool DAVID Bioinformatics resources.
For rs25531, the frequency of G-allele carriers in individuals carrying at least one L allele was 43.9% and using the functional classification proposed by Hu et al. 2006, 21.4% were low functioning, 50.0% intermediate function, and 28.6% high function genotype carriers (see Table II).
Using the functional classification scheme of the Sanger Institute (Additional File 3), we classified CDSs in functional categories.
Figure 1 shows examples of comparison between T. cruzi and H. sapiens protein structures, using the functional classification determined by AnEnΠ Figure 1(a) presents the structural alignment (R M S D C α = 0.65 Å) between the T. cruzi protein model (yellow), obtained with MHOLline, and the homologous structure (PDB 1F14) of L-3-Hydroxiacyl-CoA Dehydrogenase from Homo sapiens (blue).
We replicate the previously described increased prevalence of incidence of lifetime major depression in individuals carrying the 5-HTTLPR S allele and exposed to child abuse [Caspi et al., 2003], but this interaction was not seen when using the functional classification of Hu et al. 2006.
The top hits were used for gene annotations, and the corresponding AGI code was used for the functional classification using the gene ontology (GO) of TAIR10 (http://www.arabidopsis.org/tools/bulk/go/index.jsp).jsp
GO slim was used for the functional classification of DGHP, and the annotations were plotted using Web Gene Ontology Annotation Plot (WEGO) software [ 30].
The UniProtKB/Swiss-Prot [ 53] database was used for the functional classification of up- and down- expressed proteins.
Gene ontology (GO) annotation was used for the functional classification of up- and down-regulated genes [ 49].
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