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The seven UBC STEM faculty member volunteers who used the final protocol had not previously used the protocol and were not involved in the development process.
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Using the final protocol, we reproducibly obtained just under 50% uniquely mapping reads (out of the total number of reads that passed the Illumina pipeline quality filter).
AD and HH designed all the amplicons used in the final protocol.
This criterion is similar to that used in the final protocol of the Ocular Hypertension Treatment Study OHTSS) [ 21].
At this stage, the BRCA1 c.5074+3A>C, BRCA2 c.1834G>A, c.2971A>G, c.3807T>C and c.9458G>C variants were not detectable but subsequent amplicon redesign as used in the final protocol led to their detection and an effective sensitivity of 100%.
A pilot study, using placebo gel, followed the feasibility studies, and the results of this were used to inform the final protocol for the phase III trial [45].
As a result of these studies, we used the following final protocol.
were used in the development of the final protocol to be used in determining the distribution of arsenic in urban areas.
The final protocol used for the purification of hepatic progenitors from hESCs is depicted in Figure 4A.
Before arriving at the final protocol used for the tumor cDNA library lysate slides, many different slide surfaces, incubation conditions, and reagent concentrations were tested.
The final protocol used was as follows: Random primer 6 (5'-AGCCAGCGAA-3', 40 mM), 10 ng DNA in a PCR buffer of (final concentration) 2.5 mM MgCl2, 0.8 mM dNTP, 2 μl Platinum Taq polymerase).
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