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We used the codeml program implemented in PAML package for the selection test.
For each coding sequence, we used the codeml program of the PAML 3.15 [24] suite to conduct a likelihood ratio test between different evolutionary models.
We used the codeml program in the PAML v.3.14 [18] package to estimate the non-synonymous divergence (dN), synonymous divergence (dS), and their ratio (dN/dS) in model 0, that allows for a single dN/dS value throughout the whole phylogenetic tree.
For all analyses of positive selection we used the codeml program from the PAML package [ 16].
We used the codeml program in the PAML package [ 26] version 3.14 to perform likelihood ratio tests of positive selection.
We used the CODEML program within the PAML suite (Yang 2007) to calculate the ML-based d N/d S (denoted as ωML) for the pairwise comparison of Mb sequences obtained from the simulations.
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We conducted analyses of positive selection using the codeml program in the PAML 4.3 package [66].
Two likelihood ratio tests of positive selection were implemented using the codeml program [13], [37], [38].
Test of positive selection on single codons was performed using the codeml program in the package PAML 3.15 [49].
Tests for selection and ancestor sequence reconstruction were carried out using the Codeml program implemented in PAML [35], [36].
The data in each sliding window were analyzed using the codeml program in the paml package [13] to fit codon model M0 (one ratio).
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