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We used the MapMan package (http://mapman.gabipd.org/) as a tool to more thoroughly visualize the pathways involved in the osmotic stress response of the two genotypes.
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For pathway analysis, we mapped all DEGs using the MapMan package [ 57] with the Osa_MSU_v7 mapping file and latest pathways downloaded from the official Web site (http://mapman.gabipd.org/web/guest/mapman).org/web/guest/mapman
The mapping files for the Mapman representation were made using the Mercator web server [ 38], and the Mapman images were made using the Mapman software [ 66].
Additional functional annotations were performed using the MapMan software [ 53].
The 1,496 genes were subjected to functional annotation using the MapMan software.
Categorization of the genes using the Mapman software revealed a huge difference between the two datasets.
Major metabolic pathways were analyzed using the MAPMAN software (Thimm et al. 2004).
Second, this reduced set of genes was annotated into functional categories using the MapMan software.
Differentially expressed genes were annotated into functional groups using the MapMan software [ 42].
Functional analysis of differentially expressed genes was performed using the MapMan software tool [ 69] using ontology adapted for potato [ 70].
We then analyzed the role played by metabolic pathways using the MAPMAN software (Thimm et al. 2004; Fig. 6).
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