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M. brunneum has a worldwide distribution and is a natural pathogen of ants [ 55], yet the used strain was isolated from Austria and therefore lacks a local co-evolutionary history with all studied host ant populations.
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All used strains were originating from mono-nucleate ascospores isolated from irregular asci.
Two commonly used strains are C57BL/6J and BALB/cJ, which differ in several regards.
The previously used Physarum strain was the diploid M3b isolate.
For the current uses, strains were stored in cysteine-triptose agar (CTA; Becton Dickinson) for up to four weeks.
For assays that used strain FA1090F62por5 8, 1% NHS was used.
sir3Δ strain was used as a control.
The E. coli TG1 strain was used.
For expression, BL21[DE3] strain was used.
swf1Δ strain was used as a negative control.
The Aedes albopictus Houston strain was used for experiments.
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