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Tryptic peptides were eluted from the reversed phase column into the mass spectrometer using a linear gradient elution of 2 40% B in 40 min. For MS experiments, following mode and tuning parameters were used: Scan range: 300 2000 m/z, polarity: positive, capillary voltage: 1900 V, flow and temperature of the drying gas were 4 L/min and 325°C.
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For tandem MS acquisition we used a scan range from m/z 50 1550 with a scan time of 1.92 s and an interscan of 0.08 s and a dynamic exclusion window of 240 s.
In addition, two arguments of ASAPRatio were chosen: (1) use fixed scan range for Light and Heavy.
The powder X-ray diffractogram was performed on PANalytical's X'Pert PRO materials research diffractometer (MRD) using a scan range (2θ) of 10 90° at increments of 0.0332°.
MS1 was performed using a scan range of 295 450 m/ z, collision energy of 35, maximum injection time of 10 ms, and resolution of 6 × 10.
Samples were analyzed using a UPLC system (UPLC Acquity, Waters Ltd., Elstree, U.K). coupled online to a Q-ToF Premier mass spectrometer (Waters MS Technologies, Ltd., Manchester, U.K). in both positive and negative ion electrospray modes, using a scan range of 50−1000 m/ z and a scan time of 0.08 s.
ECL was excited using reaction with coreactant tripropylamine in aqueous solution in the anodic potential range using scanning voltammetry method.
We perform tests in an inner city park and a suburban street using a scanning range laser as the sole information source.
Computed tomography coronary angiography will be performed where possible, with a prospective gating technique using the minimum scan range planned from the unenhanced scan.
The frequencies used in the scan ranged from 106 to 10−2 Hz, and an applied voltage of 10 mV was used.
The scan range used was from 300 to 1800 m/z.
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