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As a reference method, we used restriction fragment length polymorphism.
A 'meganuclease', such as I-SceI, that recognizes and cuts longer sequences (18 bp in the case of I-SceI) than those cut by commonly used restriction enzymes.
Ready to use expression vector with eight commonly used restriction enzyme recognition sites in multiple cloning site was subsequently constructed.
In addition to using real-time PCR, we used restriction enzymes to generate differential digestion patterns that led to the development of an HLA-B*57 01 HLA-B*57 01tion fragment length PCR restrictionrker.
A lot more talent, creativity, and attention to detail goes into creating an enjoyable video, and just as Twitter did with its own product, the company has used restriction to force quality content out of its users.
In addition, numerous commonly used restriction enzyme sites exist on the backbone of the binary vector, limiting the construction strategy with regard to the use of restriction sites.
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To present the LT-ST fusions to the mucosal immune system, we used restriction-free cloning to incorporate them into the passenger domain of the autotransporter protein (EspP) expressed on a medium copy number plasmid.
Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations.
We used restriction-site-associated DNA (RAD) sequencing to investigate the population structure and demography of Chinook salmon from western Alaska.
The first such study used restriction-digest mapping of mitochondrial DNA (mtDNA) ribosomal region to find weak support (maximum parsimony bootstrap support of 57%) for the extant rhinoceros phylogeny as outlined on the basis of horn morphology [ 7].
"Gene use restriction technology has its benefits.
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