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In addition to the non-photosynthetic stramenopiles, we used representatives of the dinoflagellates, haptophytes, 'green plants', and rhodophytes as outgroups and for calibration purposes.
In addition, we used representatives of twelve heterothallic (self-sterile) species and subspecies (Additional file 2: Table S2).
As outgroups to root the tree we used representatives from Hydaticini, which are thought to be the closest relatives [ 12], and Dytiscini.
To conduct a comparative analysis of Malaysia's M. f. fascicularis, we used representatives of all M. f. fascicularis sequences available in GenBank for the mtDNA control region (CR).
As multiple outgroup, we used representatives of the clade Yinpterochiroptera Teeling et al., 2005: Hipposideros bicolor [ AF358131], Rhinolophus hipposideros [ AF044660], Rousettus leschenaulti [ AF044662] and Epomophorus wahlbergi [ AF044642].
To this end, we first used representatives of all known TA protein families and the new candidates identified with the first approach as queries for exhaustive PSI-BLAST [ 39] searches.
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Imidacloprid is the most well-known and widely used representative of the neonicotinoid insecticides.
"To me, if you own something, you ought to have a say about how and when it's used," Representative Doris Matsui, of California, said.
The FR method SSIM[94] is a commonly used representative method of this area.
The simulation used representative or non-representative aggregate protocols to evaluate the TCP performance, namely TCP and UDP protocols.
Firstly, the study used representative food samples.
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