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In some cases where standard PCR failed to amplify target loci, we used Ready-To-Go PCR Beads (GE Healthcare) following manufacturer's recommendations with markedly improved success.
Hemolysis-free serum samples were removed under sterile conditions, and indices were determined using ready to-use-Kits.
Complementary DNA was synthesized using Ready to Go You-Prime First-Strand Beads (GE Healthcare, Chalfont, UK) with total RNA (1 μg) and oligo dT) primers.
Using ready-to-serve noodles feels a bit like cheating, but it's cheaper and tastier than a takeaway, so who cares?
Mince pies are easy – unless you're a purist and make your own mincemeat – and using ready-to-roll pastry does not detract.
Bound secondary antibodies for IgG and IgM, and a third antibody group for IgG subclasses were quantified using ready-to-use TMB (3,3', 5,5'-Tetramethylbenzidine) substrate.
Using ready-to-use therapeutic foods, trained health extension workers can and do save lives.
cDNA was synthesized using Ready-To-Go T-primed first strand kit (Invitrogen, Germany).
Both probes were labelled by dCTP [P] using 'Ready-to-go' kit (Amersham).
2. Ensemble reactions of RT-PCR using Ready-to-Go RT-PCR Beads, Amersham Biosciences.
cDNA was synthesized using Ready-To-Go You-Prime the First-Strand Beads (Amersham Biosciences, Piscataway, NJ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com