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No soldering, though, we used ready PCBs (to the disgust of some).
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She happily used ready-made objects – mirrors, laboratory glassware, old clothes, bits of machinery.
Like Mr. Wesselmann, who used ready-made objects like a red telephone, Segal used a washing machine, a red plastic chair and a garbage can in his re-creation of a woman reading a book, waiting for her laundry to be finished.
In some cases where standard PCR failed to amplify target loci, we used Ready-To-Go PCR Beads (GE Healthcare) following manufacturer's recommendations with markedly improved success.
We used ready-made TaqMan® assays on the analyzed genes and custom-designed GAPDH primers and TaqMan® probe [ 52] (Applied Biosystems).
For PCR of IL-6 and TNF-α, we used ready-made Pre-Developed TaqMan® Assay Reagents and the TaqMan® 7700 instrument (Applied Biosystems).
The Americans have regularly counseled using ready bailout money as a firewall in the crisis.
You can use ready.gov to make an emergency plan for your family.
Hemolysis-free serum samples were removed under sterile conditions, and indices were determined using ready to-use-Kits.
Zymography was performed using "Ready Zymogram Precast Gel" (Biorad) (10%) gels copolymerized with 1 mg/ml gelatin.
PCR reactions were carried out in a volume of 25 μl using ready mix PCR tubes (Syntezza, Jerusalem, Israel).
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