Sentence examples for used previously designed from inspiring English sources

We used previously designed and tagged primers (reverse primer with T7-promoter tag and forward primer with 10mer tag sequence as balance) for the 10 candidate genes [17].

To generate expression vectors containing genes for fibers with zippers incorporated into an extended H-I loop, we used previously designed fiber shuttle plasmid pHI-PB40 [13] as starting material.

Due to the size and high GC content of SVA elements, we used previously designed internal PCR primers [ 27].

Based on previously described guidelines [ 44], we designed three AONs targeting different regions in MSTN exon 2 gene and used previously designed AONs targeting DMD exons as controls (Table 1).

We used previously designed primers to amplify the OtsClock1b PolyQ domain in Feather River Chinook and test for frequency differences in length polymorphisms among migratory groups (O'Malley et al. 2007).

To develop multiplex PCR systems we used previously designed HLA-A, HLA-B, HLA-C, and HLA-DPB1 locus-specific primer sets that cover the whole gene regions from the promoter-enhancer region to 3′UTR with the product size of 5.5 kb in HLA-A, 4.6 kb in HLA-B, and 4.8 kb in HLA-C, and from intron 1 to 3′UTR with the product size of 7.2 kb in HLA-DPB1 [ 31].

Yet each can be addressed individually, using previously designed techniques, and it is possible to read and write digital ones and zeros at each wire junction by electronically changing the properties of the molecules.

Real-time PCR assays targeting 3 species-specific genes, ecfX, gyrB and oprL, were developed using previously designed and new primers.

Detection of BphA1 gene (first gene of bacterial aerobic PCB degradative pathway) in genomes of rhizosphere microorganisms was performed by nested PCR technique using previously designed specific primers.

Using previously designed efficient cell-penetrating peptide NF55, we searched for strategies enabling control over the nanoparticle formation and properties to further improve transfection efficacy.

Single-nucleotide polymorphism genotypes were assayed by pyrosequencing using previously designed sequencing primers (Supplementary Table 2).