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During EDXS mapping, the used pixel dwell time was ~4.4 ms at a spatial dimension of ~0.04 nm/pixel Fig. 4 a HAADF-STEM image of a NP and its surroundings.
Since we detected no obvious differences in coelomocyte GFP contents, we instead used pixel intensity measurements for increased sensitivity.
For partially overlapping bands, we also used pixel density (for signal area) quantification with Adobe Photoshop.
Image pixel resolution was set at 1600 × 1600, and used pixel dwell time of 4 μs and one-way scanning.
Specifically, we used pixel intensity to quantify the level of GFP or mCherry expression for both wild-type and RNAi treatment for each gene.
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The popular widely used pixel-based fusion rule is the aforementioned maximum selection scheme [20].
Considering these facts, we used pixel-wise computations (2) and (5) in our simulation.
It is concluded that the object-based ensemble analysis is a promising alternative to the commonly used pixel-based biomass modeling techniques.
Therefore, in this paper, we are still concerned about pixel level fusion, and when the terms "image fusion" or "fusion" are used, pixel-level fusion is intended.
However, the inferred left-lateral strike-slip on F3 turned out to be insignificant as mentioned before; this may be because we used pixel-offset data for the inversion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com