Sentence examples for used one step from inspiring English sources

Herein, we used one step protein precipitation by combining trichloroacetic acid and methanol (9 : 1, v/v) that greatly shortened the time of analysis.

The following program was used: one step at 95°C for 10 min, 55 cycles of de-naturation at 95°C for 15 s, annealing at 65°C for 15 s and elongation at 72°C for 15 s.

The odds ratios for income were calculated using one step on the logarithmic scale.

OxZr is characterized by hard zirconium oxide (oxide) formed on Zr2.5Nb using one step thermal oxidation carried out in air.

The PCF, based on BaTiO3 and reduced graphene oxide was prepared using one step hydrothermal process.

Contrasted to traditional air-drilling process, the fatigue life of fastener hole could be improved by 44 55% using one step compound cutting process.

Citrate ions were encapsulated into Mg-Al LDH using one step co-precipitation reaction.

The quantitative real time PCR (qRT-PCR) was performed using One Step Real Time RT PCR (Applied Biosystems) with SYBR Green dye.

RNA that was isolated using the Trizol (Invitrogen) as described above was reversely transcribed using One Step PrimeScript miRNA cDNA Synthesis Kit (TaKaRa).

Briefly, 3 μg miRNA was reverse transcribed to cDNA using One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen Biotech Co., China).

The MRT-PCRs were performed using One Step RT-PCR Kit (QIAGEN, catalogue number 210212, Valencia, CA), according to Chiapponi et al. [ 35].