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The double-mutant M-1-2 was generated using primer 1 and 2 on the template cDNA obtained from mutant M-1 and primers 3 and 4 were used on the template cDNA obtained from mutant M-2.
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This was the template we used on the film, and it's reductive and catchy, certainly one way to process the gargantuan archive when you only have ninety minutes.
The Excel worksheets must conform to a standard Excel template, as used on the example spreadsheet (see Figs. 1 and 2 and "Availability of data and materials" section).
Participants then responded to some more questions on the effect the use of the template had on their understanding of their research work.
Methods have been developed for improving microsatellite PCR results from degraded DNA e.g., [19], [25] and further research might elucidate whether our stringent setup can be relaxed somewhat, and whether these novel approaches can be used on aDNA templates without increasing the risk of cross-contamination.
Primers designed for the isolated fragments were used on template from tumors and normal colonic tissue for comparison of expression.
Finally, once the model amino acids were fully substituted, insertions/deletions integrated, and the valyl-adenylate substrate added, full structural refinement (Misura et al. 2006) using constraints on the template yielded the completed model (see Figure 4).
Primers designed for COL11A1, COL5A2, WISP-1 and the internal control GAPDH were used on templates from tumors and normal tissue for comparison of expression.
Individual DNA samples were amplified using the identical primers to those used on pooled DNA templates.
Island organization of particles have been fabricated in a microfluidic device consisted of upper and lower conductive indium-tin-oxide (ITO) substrates with interdigitated microband array (IDA) electrode used as the template based on a dielectrophoresis (DEP).
Depending on the intended use of the template-switching reaction, it is therefore very important to find optimal conditions for cap-specific and cap-independent reverse transcription reactions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com