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A related algorithm based on Bloom filters has also been used on sequence data to detect and calculate the frequency of induced sun mutations in human skin (Stranneheim,S. et al. submitted for publication).
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Because these alignments rely on information about all the query sequences' three-dimensional conformations, the method can only be used on sequences where these structures are known.
RepeatMasker [ 43] was used on sequences for each of the three classes for each species in order to identify the content for repeat elements in the BESs.
To find strain-specific repeats online, the NCBI BLAST database for microbes was used on genomic sequences with sequencing status 'Complete, in Progress or Assembly'.
When used on the sequence containing the rs327 T allele, one set of MC sequences eliminated the bandshift (Fig. 4A).
The method described in this manuscript is applied to HIV but can be used on any sequence dataset.
While BLAST has been adapted by researchers to process non-biological sequences [18], Strand can be used on general sequences "out of the box" and performs more efficiently than BLAST.
To understand the differences in classification between the methods, MEGABLAST was used on all sequences uniquely classified by one method.
Note: blastpgp can only be used on protein sequences and not nucleotides.
The primers used on the sequencing reactions were the same used on the amplification of the target fragments.
In a second step, the full energy model can be used on sub-sequences to refine potential binding sites.
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