Three mice were used for negative control.
A "morphologic tumor activity" (MTA) score, determined by light microscopy, was assigned within each of 322 randomly chosen ROIs, on negative control slides, using a scale of 1 (inactive), 2 (probably inactive), 3 (probably active), and 4 (active).
AAV-TRE-EGFP&AAV-Tet-On and AAV-CAG-TRAIL were used as negative control and positive control, respectively.
Solvent and Ningnanmycin which were smeared on other plants were used as negative control (CK−) and positive control, respectively.
The background strain CEN.PK 113-7D was used as negative control for growth on d-xylose (first row).
Cycloheximide (serial dilutions starting at 1000 μg mL−1) as well as the serial dilutions of 96% EtOH were integrated on changing positions, and used as negative control (AB susceptibility control) and as solvent inhibition control, respectively.
We used a negative control inhibitor based on Caenorhabditis elegans miRNAs not found in humans (miRIDIAN miRNA hairpin inhibitor negative control).
No specific bands were obtained when we performed Western blot analyses: (i) on lysates from LNCaP, used as negative control [ 36], (ii) by omitting the primary antibody and (iii) by blocking the primary antibody with the TRPC6 peptide.
MiR-205 mimics, designed to mimic endogenous mature miR-205, were purchased from GenePharma (Shanghai, China) as well as scrambled oligonucleotides, which did not produce identifiable effects on miR-205 function, used as negative control miRNA.
Human GAPDH control On-target SMART pool siRNA (# D-0018301020, Dharmacon) was used as negative control.
