Exact(3)
We used Mission shRNA lentiviral transduction system, which includes sequence-verified shRNA lentiviral plasmids (Sigma Aldrich).
For the ESR1 and VAV3 expression depletion assays, we used MISSION shRNA (Sigma-Aldrich).
For FLG2 knockdown in keratinocytes, we used MISSION pLKO.1-puro vector based lentiviral particles containing a puromycin resistance gene and a shRNA insert under the U6 promoter (Sigma-Aldrich, St . Louis MO, USA).
Similar(57)
The important role of using mission, vision and strategy has been acknowledged in most organizations today.
The system uses "mission cards" that friends pass to each other along with a mission i.e. give someone a book or buy someone a meal.
You may remember Akoha, a startup which launched at the 2008 TechCrunch50 conference which uses "mission cards" that friends pass to each other along with a mission ( i.e. give someone a book or buy someone a meal).
Companies use mission statements to align, inspire, and inform their team and their clients.
The game's story is told using mission briefings and animated cutscenes.
There is an DOS policy that prevents contractors from using mission vehicles and personnel for leave rotations.
We have also confirmed our plasmid silencing data using Mission® lentiviral shRNA transduction particles to silence RASSF1C expression in T47D cells.
PITX2 expression was stably silenced in MDAMB231 cell line, which had high endogenous levels of all three PITX2 transcript isoforms, using Mission lentiviral transduction particles (Sigma, St . Louis and selecting the stably transduced cells with puromycin.
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