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Our method is suitable for on-site screening, whereas the commonly used method is not.
The subtenon approach is clearly less invasive than the intravitreal one [ 39] although, here again, this commonly used method is not free of potential complications such as the accidental injection directly into the choroidal or retinal circulation, perforation of the ocular bulb, occlusion of the central retinal artery and cataract [ 39].
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Since in the majority of cytokine studies analyzed here the sensitivity of the used method was not given it is not clear if the many negative results reported are "real" or due to low method sensitivity.
Mechanical exfoliation, one of the most commonly used methods, is not favorable for commercial applications.
And many currently used methods are not convenient for on-site testing or not able to distinguish water from the metal particles.
Ethical approval signed by the Semmelweis University Regional and Institutional Committee of Science and Research Ethics was not required for the study because all used methods were not more than normally needed for diagnosis and treatment.
Distilled water lavage might induce tumour cells lysis, but the currently used method is probably not sufficient to achieve this beneficial effect.
The imposed complexity due to using such method is not cleared in this study.
Unfortunately, quantification of virus using this method is not as sensitive as plaque assays and molecular techniques (real time PCR).
Using this method is not perfect but it works, and makes for a fun treat that kids will love!
Since the velocity data measured used the above method is not quite reliable or not mature, we disregard it.
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